Gene-Based Stem Cell Therapy Specifically Removes Cell Receptor That Attracts HIV

UCLA AIDS Institute researchers successfully removed CCR5 - a cell receptor to which HIV-1 binds for infection but which the human body does not need - from human cells. Individuals who naturally lack the CCR5 receptor have been found to be essentially resistant to HIV.

Using a humanized mouse model, the researchers transplanted a small RNA molecule known as short hairpin RNA (shRNA), which induced RNA interference into human blood stem cells to inhibit the expression of CCR5 in human immune cells.

The findings provide evidence that this strategy can be an effective way to treat HIV-infected individuals, by prompting long-term and stable reduction of CCR5.

Saki Shimizu, Patrick Hong, Balamurugan Arumugam, Lauren Pokomo, Joshua Boyer, Naoya Koizumi, Panyamol Kittipongdaja, Angela Chen, Greg Bristol, Zoran Galic, Jerome A. Zack, Otto Yang, Irvin S.Y. Chen, Benhur Lee and Dong Sung An, all of UCLA.

Blood, Journal of the American Society of Hematology

The research was funded by a Rheumatology Fellowship Training grant, the UCLA AIDS Institute, the UCLA Center for AIDS Research, the National Institute of Allergy and Infectious Diseases, the National Heart, Lung and Blood Institute, and the National Cancer Institute.

Source:
Enrique Rivero
University of California - Los Angeles ational Cancer Institute.

MAKING MONEY BY THE CLICK

Forget looking for your next job in a traditional office. Armies of recovering cubicle dwellers are making an honest-to-goodness living right here on the Web -- and we don't mean by selling diet pills or kitchen accessories to unsuspecting friends and relatives.
From writing and graphic design to software development and social media marketing, countless creative types are hanging their own virtual shingle, often with a minimum of overhead, sometimes even surpassing the salary they made as an employee.

Seven successful web workers share how they did it, how you can follow in their footsteps, and what pitfalls to watch out for:

1. Blogger

According to the Wall Street Journal, 1.7 million Americans make money blogging and 452,000 of them derive a majority of their income from it. Ariel Meadow Stallings is one such blogger, dividing her time between the blog she writes for her part-time corporate job and her own blog, Offbeat Bride. "It took about a year to build traffic to the point where advertising and sponsorships made sense," says Stallings, who's been publishing OffbeatBride since January 2007 and now averages nearly a million page views a month. Her advice to would-be bloggers? "Just blog. And then blog more. And read other blogs." For tips galore on earning a living as a blogger, see ProBlogger.

2. Web Designer

Bloggers often need help updating their site design and code, which is where freelance web designers like Liz Andrade of CMD+SHIFT Design come in. Like many Web jobs, once you have the skills in the bag, the office overhead is minimal. "If I have a laptop and an Internet connection, I'm in business," Andrade says. And while she studied design in college, she learned far more about Web design and business branding on the job. To follow in her footsteps, Andrade suggests first working in a junior position at a boutique design firm. She also suggests meeting other freelance designers on at popular online water coolers like FreelanceSwitch.com, FreelanceFolder.com, and WebWorkerDaily.com.

3. Social Media Marketer

According to the Pew Internet and American Life Project, 1 in 10 U.S. adults now use microblogging sites like Twitter to update friends and colleagues on their status, mood, or latest personal news. Not surprisingly, publicists and marketing mavens like Lisa Hanock-Jasie have begun promoting their clients on these social media sites. "It's become more a virtual work existence these days, with the majority of client contact via the Web -- be it live Web chat, e-mail, or IM -- or phone conversation," the independent publicist says. The upside: finding work online is easier than ever. The downside: the isolation. To beat the solitude, Hanock-Jasie regularly schmoozes with several professional groups on LinkedIn.

4. Web Community Founder

With the Pew Internet and American Life Project reporting that a majority of U.S. adults under age 70 use the Internet, it's no surprise that Web communities have been sprouting up like weeds. But launching a successful online community is much more involved than starting a freelance business, says Lara Eve Feltin, who in 2005 co-founded Biznik, an award-winning web community for the self-employed, with husband Dan McComb.

"Dan built the first version of the site in his spare time," Feltin says. "And because our product helps small businesses, a lot of people offered to help us for free." Dan's Web development work alone saved them hundreds of thousands of dollars, Feltin says. In short, be prepared for lots of ramp-up time and -- if you have no volunteers -- lots of programming and legal expenses.

5. E-commerce Site Owner

Armies of savvy craftsters and artists sell their wares online. Take Maggie Kleinpeter and Michael Pittard, who in 2001 opened the online store Supermaggie, where they sell Kleinpeter's hand-made scarves and print T-shirts to retail and wholesale customers. "It took about three years for it to provide full-time income for both of us," says Pittard, who built and maintains the website. The pros of running an online boutique: "The world is your storefront, and we have practically no overhead," says Pittard. The cons: There are no dressing rooms online, and customer feedback isn't instantaneous. To learn more about starting your own e-commerce business, see the community pages on Etsy.com and sites like TheSwitchboards.com, SuperNaturale.com, and Craftster.org.

6. Social Media Application Developer

In March 2009, the blog TechCrunch.com ran the headline "Some Indie Facebook Developers Pulling in over $700,000 a Month." While Web developer Jesse Stay has yet to hit that financial jackpot, he does credit SocialToo -- the online collection of social media tools and services he built -- with providing 80 percent of his income. (The rest comes from consulting work.)

His advice for hopeful social media developers? "Don't expect to get any investment." Most startups never see a venture-capital dime, even in good financial times. Instead, he says, you'll need to rely on your savings until you make enough cash to pay yourself a salary. To learn more, see the blogs All Facebook and Inside Facebook and the developer forums and wikis of your favorite social media platforms.

7. Infopreneur

You know that old saying about making money in your sleep? There's an entire population of Web entrepreneurs who aren't just waxing poetic about it -- they're doing it. Erin Blaskie, an Internet marketing specialist who was making six figures by age 23, is one of them. Though she got her entrepreneurial start in 2004 as a virtual assistant, she now creates and sells virtual training programs for entrepreneurs who want to promote themselves online.

Her advice to hopeful infopreneurs? "Check out what already exists in your market and see where there are gaps." Also, "Ditch the perfectionism! The cool thing with info-products is you can produce it, publish it, and edit it later if need be."

Source:
http://hotjobs.yahoo.com/career-articles-web_2_0_careers_making_money_by_the_click-902

AGB MEGA MANILA TV RATINGS (FEB. 23-25):AGUA BENDITA GRABS LEAD IN THE PRIMETIME RACE

Here are the comparative TV ratings of ABS-CBN and GMA-7 programs from February 23 to 25, based on the overnight ratings of AGB Nielsen Philippines among Mega Manila households:

February 23, Tuesday (Incomplete data)
Daytime:
Diz Iz It! (GMA-7) 7%; Showtime (ABS-CBN) 19.6%
Eat Bulaga! (GMA-7) 19%; Daisy Siete: Bebe & Me (GMA-7) 13.7%; Wowowee (ABS-CBN) 20.6%
Gumapang Ka Sa Lusak (GMA-7) 12.7%; Magkano ang Iyong Dangal? (ABS-CBN) 14%
Ina, Kasusuklaman Ba Kita? (GMA-7) 15.8%; Maria de Jesus: Anghel Sa Lansangan (ABS-CBN) 11%
Laff En Roll (GMA-7) 9%; Banana Split Daily Servings (ABS-CBN) 8.2%; Pilipinas Got More Talent (ABS-CBN) 9.1%
Family Feud (GMA-7) 12.4%; The Substitute Bride (ABS-CBN) 12.4%

Primetime:
First Time (GMA-7) 21.4%; Tanging Yaman (ABS-CBN) 19%
24 Oras (GMA-7) 28.6%; TV Patrol World (ABS-CBN) 25.9%
Panday Kids (GMA-7) 31.6%; Agua Bendita (ABS-CBN) 33.4%
The Last Prince (GMA-7) 29.7%; Kung Tayo'y Magkakalayo (ABS-CBN) 32.2%
Full House (GMA-7) 28.1%; Habang May Buhay (ABS-CBN) 29.9%; Rubi (ABS-CBN) 23.8%
Queen Seon Deok (GMA-7) 21.3%; SNN: Showbiz News Ngayon (ABS-CBN) 14.1%

February 24 (No available data)

February 25, Thursday (Incomplete data)
Daytime
Diz Iz It! (GMA-7) 7.4%; Showtime (ABS-CBN) 21.9%
Eat Bulaga! (GMA-7) 19.3%; Daisy Siete: Bebe & Me (GMA-7) 12.2%; Wowowee (ABS-CBN) 20.6%
Gumapang Ka Sa Lusak (GMA-7) 12%; Magkano ang Iyong Dangal? (ABS-CBN) 12.7%
Ina, Kasusuklaman Ba Kita? (GMA-7) 14.6%; Maria de Jesus: Anghel Sa Lansangan (ABS-CBN) 7.8%; Banana Split Daily Servings (ABS-CBN) 7.4%
OC To the Max (GMA-7) 8.1%; Pilipinas Got More Talent (ABS-CBN) 8.1%
Family Feud (GMA-7) 9.8%; The Substitute Bride (ABS-CBN) 11.5%

Primetime:
First Time (GMA-7) 19.9%; Tanging Yaman (ABS-CBN) 17.8%
24 Oras (GMA-7) 28.9%; TV Patrol World (ABS-CBN) 25.9%
Panday Kids (GMA-7) 33.2%; Agua Bendita (ABS-CBN) 34.3%
The Last Prince (GMA-7) 32.2%; Kung Tayo'y Magkakalayo (ABS-CBN) 30.3%
Full House (GMA-7) 29.3%; Habang May Buhay (ABS-CBN) 27.5%; Rubi (ABS-CBN) 23.5%
Queen Seon Deok (GMA-7) 21.8%; SNN: Showbiz News Ngayon (ABS-CBN) 14.6%
SRO Cinemaserye Presents Meet the Fathers (GMA-7) 12.5%; Bandila (ABS-CBN) 9.4%


Source:
http://www.pep.ph/news/24838/AGB-Mega-Manila-TV-Ratings-(Feb.-23-25):--Agua-Bendita--grabs-lead-in-the-primetime-race